Pre-clinical appraisal of Panchashirisha Agada for anti-microbial activity - An in-vitro trial

Abstract

Introduction: The continuous emergence of multidrug resistance microbes and fungus and negative impact of synthetic preservatives have led to an increased interest in the use of plant extract as alternative antimicrobial agents. In the present investigation antimicrobial activity of Ayurvedic formulation Panchashirisha agada has been evaluated against human pathogenic bacteria and fungus. The drug Shirisha (Albizia lebeck Benth) is the major constituent in Panchashirisha agada. The drug Shirisha (Albizia lebeck Benth) is having potent antimicrobial activity and is proven by many research studies. The drug is mentioned in charaka samhita as best vishagna, antidote in Ayurveda. Aims and Objective: 1) Phytochemical analysis of Panchashirisha agada 2) To evaluate anti-microbial activity of Panchashirisha agada. Methodology: Analysis of antibacterial activity of extract is determined by using Agar well diffusion technique for following organisms S. aureus, S.pyogens, E.coli, P.aeroginosa. Determination of antifungal activity is done by using cup plate method and minimum inhibition concentration for organism Candida albicans and Aspergillus. Result: The result from this study indicated that S. pyogens was the most sensitive bacteria to the Panchashirisha agada extract with lowest MIC concentration 50mgl/ml. The Panchashirisha agada extract didn’t show any inhibition against Candida albicans and aspergillus. Conclusion: The study revealed that the Panchashirisha agada possess antibacterial activity in a dose dependent manner. The antimicrobial property may be due to presence of several saponins.