Pepsin modulation activity of Truptighna Dashemani drugs in correlation with total phenols: An in-vitro study
DOI:
https://doi.org/10.70066/jahm.v14i6.2667Keywords:
Dyspepsia, pepsin activation, pepsin inhibition, trupti, phenolic contentAbstract
Background: Trupti is early satiety and likened to Dyspepsia which occurs as a symptom in many gastro-intestinal conditions. Functional dyspepsia (FD) presents with bothersome post prandial fullness, pain, burning in the epigastrium and early satiety. Pepsin enzyme has been recently identified as one of the potential targets in FD. Charaka Samhita mentions Truptighna Dashemani, for treating the condition Trupti (Dyspepsia). Objectives: The present study aimed to explore Pepsin enzyme modulation activity of Truptighna Dashemani drugs and relate this with the phenolic content present in them. Methods: Hydro alcoholic extract of each drug of Truptighna dashemani was prepared by cold maceration. Estimation of total phenols was performed by Folin-ciocalteu method using Gallic acid as standard. Pepsin enzyme assay was performed following internationally accepted standard INFOGEST protocol and was then correlated with total phenols in each drug. Results: Chavya showed the highest phenolic content (109.88 ±0.29 mg GAE/ gm of extract) while Pippali and Vacha showed the least. Guduchi showed the highest Pepsin activity (23633 units/ mg) followed by Pippali (22633), Chitraka (17533 units/ mg) and Vidanga (12933). Patola and Nagara showed negative values. Kruskal Wallis ANOVA comparing the results with positive control showed significant differences in Pepsin activity among eleven groups (control + ten extracts; H = 27.6, P = 0.0026, n = 3 per group). The association between phenol content and pepsin activity was assessed using nonparametric Spearman correlation, revealing moderate negative correlation (r = −0.56, P = 0.096, n = 10) indicating greater pepsin inhibition by extracts with lower phenolic content. Conclusion: Truptighna Dashemani drugs have Pepsin modulation action as evidenced by invitro studies. The total phenolic content present in the individual drugs might be having role in Pepsin activity modulation. Drugs showing Pepsin activation can act as digestive stimulants while those inhibiting pepsin action act as gastro protectives.
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